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Blue White

Why is it a colony on the plate, both the blue (inside) and white (outside)? Blue / white selection?.

Hi, this is for blue / white selection. colonies and expected to be white or blue. but surprisingly I have a few colonies that were both. What is the explanation behind? Thank you for the great input.

Perhaps the colony becomes too long and the short of X-gal on the edges? I do not think this is due to the reversal (loss of expression of b-galactosidase), but also possible and perhaps also because the plate was incubated long.

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